Plasmid Creation and Cell Line Generation
The journey begins with the creation of plasmids harboring the genetic blueprint for the desired proteins. Our company excels in this crucial step, leveraging advanced techniques in molecular biology and bioinformatics to design rational constructs tailored to meet the unique requirements of each client's research goals. Our team of experts carefully design plasmids suitable for expression in a variety of host systems, ensuring maximum protein production and fidelity.
Protein Expression and Purification
Once the plasmids are engineered, the next step involves the expression and purification of the target proteins. Our company's state-of-the-art facilities and experienced team enable seamless protein expression across a variety of expression platforms, including Escherichia coli, mammalian cells, and baculovirus/insect cells. Through rigorous optimization protocols and cutting-edge purification techniques, we guarantee the production of high-quality proteins suitable for downstream applications, including in vitro assays and crystallization studies.
Protein Crystallization
Protein crystallization marks a pivotal stage in the structural biology pipeline, facilitating the determination of three-dimensional protein structures. Our company's expertise in crystallization spans both novel and published targets, with a proven track record of success in generating high-quality crystals amenable to X-ray diffraction studies. Our dedicated team employs a diverse array of crystallization techniques, including vapor diffusion, microbatch, and sitting drop methods, tailored to each target's unique characteristics.
Crystal Structure Determination and Analysis
With crystals in hand, our company embarks on the journey of crystal structure determination and analysis. Leveraging our access to synchrotron beamlines and cutting-edge X-ray diffraction facilities, we collect high-resolution diffraction data, enabling the precise determination of atomic coordinates and elucidation of protein structures. Our team of structural biologists employs advanced computational tools and software for structure refinement and analysis, unraveling the intricate details of protein-ligand interactions and functional mechanisms.
Generation of Small Molecule Complexes
In addition to elucidating the structures of individual proteins, our company specializes in the generation of small molecule complexes with proteins through soaking and co-crystallization techniques. By harnessing the power of structural biology, we provide invaluable insights into the binding modes and interactions of small molecules with their biological targets, guiding rational drug design efforts and facilitating the development of novel therapeutics.
