In Vitro Biology Services

Biophysics and Other Binding Assays

Our biophysics and binding assays play a crucial role in characterizing the interactions between molecules, providing valuable insights into binding affinity, kinetics, and mechanism of action. Through techniques such as surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and fluorescence polarization (FP), we assess molecular interactions with precision and accuracy.

Cell Functional/Phenotypic Screening

Our cell functional and phenotypic screening assays enable the evaluation of compound efficacy in cellular models relevant to disease biology. By assessing functional endpoints and phenotypic changes, we identify lead compounds with therapeutic potential and elucidate their mechanisms of action.

Target Engagement Assays

Our company's target engagement assays provide critical insights into the interaction between drug candidates and their intended targets in complex cellular environments. Through innovative techniques such as proximity-based assays and cellular thermal shift assays (CETSA), we assess target engagement and guide lead optimization efforts.

Mechanism of Action Studies

Understanding the mechanism of action is essential for optimizing drug candidates and predicting their efficacy and safety profiles. Our comprehensive approach to mechanism of action studies encompasses a range of techniques, including molecular profiling, pathway analysis, and functional genomics, to elucidate the intricate mechanisms underlying drug activity.

Selectivity Panel Evaluation

Assessing compound selectivity is crucial for minimizing off-target effects and optimizing safety profiles. Our company offers comprehensive selectivity panel evaluation services, utilizing a panel of targets relevant to the therapeutic area of interest to assess compound specificity and selectivity.

Target Science

• Our company excels in target discovery and validation, leveraging cutting-edge technologies and expertise to identify and validate novel drug targets.
• Additionally, our expertise in cell line engineering enables the development of robust cellular models tailored to specific drug discovery programs.

Hit Identification and Hit-to-Lead Optimization

• Our hit identification services encompass assay development, fully automated high-throughput screening, and DNA-encoded library (DEL) screening, enabling rapid identification of lead compounds with desirable pharmacological properties.
• Subsequent hit-to-lead optimization involves biophysics, structural biology, and biochemical and cellular activity assays to refine lead candidates for further development.

Lead Optimization and Candidate Selection

• Our company offers a comprehensive suite of services for lead optimization, including cellular phenotype analysis, translational cell and tissue models, and signaling pathway exploration. Our focus on selectivity and safety ensures the identification of lead candidates with optimal efficacy and minimal off-target effects.
• Furthermore, our expertise in off-target and safety assessment, mode of action elucidation, and PD (pharmacodynamic) biomarker profiling facilitates informed candidate selection decisions, guiding successful drug discovery programs.